Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214726_iu3x AATAAGCCAGGCATGGTGCCTGGGGGCAGGAG(+ATATAT)GATCCTGACTAGAAAGCGATTGGTCTGCATA NGG 10 8 17 8 24 0 100 5 10
    240606214725_nf3a CTCACTATGCAGACCAATCGCTTTCTAGTCAG(+ATATAT)GATCCTCCTGCCCCCAGGCACCATGCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606214721_qhwp GTGTGTGTGTGTAAACCAGAGGGAAACCCCAG(+ATATAT)GTACCAGTCCTCTGATGCTGTCTACTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606214720_pbsb TGCAGGAAAGAGTAGACAGCATCAGAGGACTG(+ATATAT)GTACCTGGGGTTTCCCTCTGGTTTACACACA NGG 10 8 17 8 24 0 100 5 10
    240606214718_dxt0 ACCAGACATGTTCTAAGGCTGCAGTGCTGCAG(+ATATAT)GTACCCTCACCTTGGAGGTCATGCTGGTGTT NGG 10 8 17 8 24 0 100 5 10
    240606214716_k70i TCTAGAACACCAGCATGACCTCCAAGGTGAGG(+ATATAT)GTACCTGCAGCACTGCAGCCTTAGAACATGT NGG 10 8 17 8 24 0 100 5 10
    240606214713_78ff TTCTTGGCATGAGCCTATCCTTATCGCCCTTG(+ATATAT)GATCCCAGTTTAACAAGACCTTTTTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240606214711_ndgb ATTGTCTTTAGAAAAAGGTCTTGTTAAACTGG(+ATATAT)GATCCAAGGGCGATAAGGATAGGCTCATGCC NGG 10 8 17 8 24 0 100 5 10
    240606214710_7r0c TGCATCTTCATCTTCATCACGACACCAGACAG(+ATATAT)GATCCAATCTTGGATGGAGACATTACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606214708_9l3s CCCTTCACTGGTAATGTCTCCATCCAAGATTG(+ATATAT)GATCCTGTCTGGTGTCGTGATGAAGATGAAG NGG 10 8 17 8 24 0 100 5 10