Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214633_mm26 GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+ATATAT)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606214631_ys7d ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+ATATAT)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240606214628_r8j2 TCCCTGCTCAAATTATGCCTCTTAGGATAAAG(+ATATAT)GTACCCAGCATTTTGTTTTGGGTGTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606214626_7379 ACTGTATTTTCACACCCAAAACAAAATGCTGG(+ATATAT)GTACCTTTATCCTAAGAGGCATAATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606214625_u55m AACACCTTTGAAAAAAAAAAAAAAAGCCTACG(+ATATAT)GTACCACTTTTATACATAGAACCCACTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606214623_miqy CCTGGGTTAAGTGGGTTCTATGTATAAAAGTG(+ATATAT)GTACCGTAGGCTTTTTTTTTTTTTTTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606214618_939l CCATTGTTCCAAGGCTGCACATGGCTTGGAAG(+ATATAT)GATCCTGTTTGAGTGTATGCTTCCTCACTCA NGG 10 8 17 8 24 0 100 5 10
    240606214616_gk3z GGAGCTGAGTGAGGAAGCATACACTCAAACAG(+ATATAT)GATCCTTCCAAGCCATGTGCAGCCTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606214615_zc1t ACTAGGAACAACTAGTTGAGCAAGTCTTTAAG(+ATATAT)GTACCGATTGTCAAAATACATCACTAAGACA NGG 10 8 17 8 24 0 100 5 10
    240606214613_gjny TAATTTGTCTTAGTGATGTATTTTGACAATCG(+ATATAT)GTACCTTAAAGACTTGCTCAACTAGTTGTTC NGG 10 8 17 8 24 0 100 5 10