Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214524_jfjq GAGCTCCATTTTTACGCTAGGCATCCATCTCG(+ATATAT)GTACCCACTAAGCCTTTATCTCTGACTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606214521_216o AGGGAGTGGAGTCAGAGATAAAGGCTTAGTGG(+ATATAT)GTACCGAGATGGATGCCTAGCGTAAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240606214520_gxj9 TCGACATGACTGTCCTTCATATGGTTGGAAAG(+ATATAT)GTACCCACAGGTTCTTCACCCCTTCTGTGTT NGG 10 8 17 8 24 0 100 5 10
    240606214518_tczx GTATAAACACAGAAGGGGTGAAGAACCTGTGG(+ATATAT)GTACCTTTCCAACCATATGAAGGACAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606214516_8f6u CCCTCCACCACTCCTGCGTGCGCATGCCTGTG(+ATATAT)GTACCTTACAATCACCCCCCCCCCCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240606214514_oowa AATATTGTGAAGGGGGGGGGGGTGATTGTAAG(+ATATAT)GTACCACAGGCATGCGCACGCAGGAGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606214512_lxek CAAACATTGTTTACCTTGTGTTTCACCCTTGG(+ATATAT)GTACCACCCATCTACAAGTAGAAATCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240606214509_8uo7 GCCCAGTACTGATTTCTACTTGTAGATGGGTG(+ATATAT)GTACCCAAGGGTGAAACACAAGGTAAACAAT NGG 10 8 17 8 24 0 100 5 10
    240606214507_81r7 GGTGCTGCCCATGATACGGCACAGCCACAGTG(+ATATAT)GTACCAGCATTGCTGCCATGTTGGCCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606214506_cxvi CTGGCTGGATGGCCAACATGGCAGCAATGCTG(+ATATAT)GTACCACTGTGGCTGTGCCGTATCATGGGCA NGG 10 8 17 8 24 0 100 5 10