Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214504_c8og AGCTTCCTCTCTGTAGTTCCCTGAGGTGGGAG(+ATATAT)GTACCCACTTTGCCATGCATAACTCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240606214502_86y2 GGAAGAGAGATGAGTTATGCATGGCAAAGTGG(+ATATAT)GTACCTCCCACCTCAGGGAACTACAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606214501_uu6s CCTGTGCCCACTGGAGCCAGAAGAGGGCATTG(+ATATAT)GATCCTGAAACTGAAGTTACAGATGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606214459_qelz GTGGCTCCCAACATCTGTAACTTCAGTTTCAG(+ATATAT)GATCCAATGCCCTCTTCTGGCTCCAGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606214457_7lpp AGGGCTGAGAAGGTAGGAGCAGTACTGCAGGG(+ATATAT)GATCCTAAGCCAGAGGTCTGGTTAATTTACA NGG 10 8 17 8 24 0 100 5 10
    240606214456_gg9d TATGGTGTAAATTAACCAGACCTCTGGCTTAG(+ATATAT)GATCCCCTGCAGTACTGCTCCTACCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606214455_kzup TCCTCTGAACTCTCTCATCTACCTGAAAACTG(+ATATAT)GATCCACAGAACAGTTAACTTTGATCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606214453_b7ab CTCAGGGTGGGATCAAAGTTAACTGTTCTGTG(+ATATAT)GATCCAGTTTTCAGGTAGATGAGAGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606214452_ulu7 ACCAGTGTTCTTTATACACTCTATTCCAGGTG(+ATATAT)GATCCTTTGACATTTACGTGGTTTGGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606214450_hcik ATGCCACCAACCAAACCACGTAAATGTCAAAG(+ATATAT)GATCCACCTGGAATAGAGTGTATAAAGAACA NGG 10 8 17 8 24 0 100 5 10