Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214449_tccg AGTCCAAAAGTCACTCTGAGATCCAAGCTTCG(+ATATAT)GATCCCCCAGACAGTGGCATCACCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606214446_5c1m ATGCAAGACAGAGGTGATGCCACTGTCTGGGG(+ATATAT)GATCCGAAGCTTGGATCTCAGAGTGACTTTT NGG 10 8 17 8 24 0 100 5 10
    240606214445_nrlp TTTTTCTTGAGCAGTAAACTGCAATTTTCTAG(+ATATAT)GATCCTTTGCTGTGGACACATGCCCATCATC NGG 10 8 17 8 24 0 100 5 10
    240606214443_uhk7 TGATGGATGATGGGCATGTGTCCACAGCAAAG(+ATATAT)GATCCTAGAAAATTGCAGTTTACTGCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606214442_nvyd GAATGTCAACTGTTCATTTTGTTGCTGCATAG(+ATATAT)GTACCATTGAGGGGGCACACTGGAATGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606214440_14ch GGACAAGCACATTCCAGTGTGCCCCCTCAATG(+ATATAT)GTACCTATGCAGCAACAAAATGAACAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606214439_j9po AAACTTGTCTAACAAGATGTTGGCTCCATCAG(+ATATAT)GTACCCCCATGTCATACCTCTGAGGCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606214437_uyfj TTAAATCAAAGCCTCAGAGGTATGACATGGGG(+ATATAT)GTACCTGATGGAGCCAACATCTTGTTAGACA NGG 10 8 17 8 24 0 100 5 10
    240606214435_mbzd ATGCTAAGAACATTTTCTACACTGAAGATTTG(+ATATAT)GATCCGATACAGGTTCTGCTTAAGAAACTGC NGG 10 8 17 8 24 0 100 5 10
    240606214434_0uja GGAAAGCAGTTTCTTAAGCAGAACCTGTATCG(+ATATAT)GATCCAAATCTTCAGTGTAGAAAATGTTCTT NGG 10 8 17 8 24 0 100 5 10