Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214336_ys6q GAATGTTAGATATTTTTTCCATGGGGTCAAAG(+ATATAT)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606214335_bzk8 CCCTATGTTTCCACTCGCAATCATATACTTAG(+ATATAT)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240606214333_u963 TAGTGACCGGTGCACACTGCAGCTACCCAGTG(+ATATAT)GTACCCCTGGAAGGGACCTGTGATGTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606214330_w7k7 TTTAACTTTGACATCACAGGTCCCTTCCAGGG(+ATATAT)GTACCACTGGGTAGCTGCAGTGTGCACCGGT NGG 10 8 17 8 24 0 100 5 10
    240606214328_dpif GGACTCCTCAGACCATGGCTGTGTGCAGCTGG(+ATATAT)GTACCAGAATCCTGTGCTCAGTCTCTGTTCG NGG 10 8 17 8 24 0 100 5 10
    240606214327_6ydu CAACACGAACAGAGACTGAGCACAGGATTCTG(+ATATAT)GTACCCAGCTGCACACAGCCATGGTCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606214325_y7mg ATCATGGAAGGCATGCCAAGTCCACCCGCTGG(+ATATAT)GATCCAGCGGGGCACAGTACAGTTCCTCCGG NGG 10 8 17 8 24 0 100 5 10
    240606214323_t1n9 AGGAGCCGGAGGAACTGTACTGTGCCCCGCTG(+ATATAT)GATCCCAGCGGGTGGACTTGGCATGCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606214322_7312 ACTAGGCAGAGCAAACATCAGGAAGTGGGAAG(+ATATAT)GTACCGATATGAAATTAAGAGAAAGGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240606214320_fyyh TTTGAGTCGGCCTTTCTCTTAATTTCATATCG(+ATATAT)GTACCTTCCCACTTCCTGATGTTTGCTCTGC NGG 10 8 17 8 24 0 100 5 10