Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214319_hth4 CCCTTGGTCCCTAATCAGTGCCTGCTGGCGTG(+ATATAT)GATCCCAGAGGAGCCTCTGTACATGCCATCC NGG 10 8 17 8 24 0 100 5 10
    240606214317_whxu ACTCTGGATGGCATGTACAGAGGCTCCTCTGG(+ATATAT)GATCCACGCCAGCAGGCACTGATTAGGGACC NGG 10 8 17 8 24 0 100 5 10
    240606214316_da49 CATGCAATAAATGACCATTACAGGTTTCAAAG(+ATATAT)GTACCCTAGGTTTTCTAGGTGAGAGTTCACA NGG 10 8 17 8 24 0 100 5 10
    240606214313_yk99 GTGGGTGTGAACTCTCACCTAGAAAACCTAGG(+ATATAT)GTACCTTTGAAACCTGTAATGGTCATTTATT NGG 10 8 17 8 24 0 100 5 10
    240606214312_otsc GGGCTGAGTGGAGACCCAGTTGGAGGAGGCTG(+ATATAT)GATCCTTTGTTAGGAAGGCAAAGGCCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240606214311_s2ky AGGTTCCCCGGGCCTTTGCCTTCCTAACAAAG(+ATATAT)GATCCAGCCTCCTCCAACTGGGTCTCCACTC NGG 10 8 17 8 24 0 100 5 10
    240606214309_g4q3 TTATCAGCTTTCATTCAGTCTTCCTTTCTCTG(+ATATAT)GATCCAATTATCTCACCTCCTTTAGCTATTC NGG 10 8 17 8 24 0 100 5 10
    240606214308_jxhy TTATGGAATAGCTAAAGGAGGTGAGATAATTG(+ATATAT)GATCCAGAGAAAGGAAGACTGAATGAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606214306_uaku CTACAATTCTATTTTAATCAGCTTTTTGGCTG(+ATATAT)GTACCTACTGCTATACCACCTAGTATGACAT NGG 10 8 17 8 24 0 100 5 10
    240606214305_cm8s CCGAAATGTCATACTAGGTGGTATAGCAGTAG(+ATATAT)GTACCAGCCAAAAAGCTGATTAAAATAGAAT NGG 10 8 17 8 24 0 100 5 10