Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214303_n3pk TTTTTTATTCCTTCTCTGGCAATTCTTTCCTG(+ATATAT)GATCCTCTGCTTAGCGAAATTATACCTACAG NGG 10 8 17 8 24 0 100 5 10
    240606214301_lvq7 TTCCACTGTAGGTATAATTTCGCTAAGCAGAG(+ATATAT)GATCCAGGAAAGAATTGCCAGAGAAGGAATA NGG 10 8 17 8 24 0 100 5 10
    240606214300_vo5r AACACGGTCAGAGGCTCCCCAGAGCTGTGGTG(+ATATAT)GTACCACCCATGTCTGAGATACTCTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606214258_lxf7 ACAGGTCCAACAGAGTATCTCAGACATGGGTG(+ATATAT)GTACCACCACAGCTCTGGGGAGCCTCTGACC NGG 10 8 17 8 24 0 100 5 10
    240606214257_4ndr AGGAGGAGGGTTTTTTTTTTAAGACAAAGATG(+ATATAT)GTACCATGGTATCAGAAAATAGTGTATAAAT NGG 10 8 17 8 24 0 100 5 10
    240606214255_0k0u AAGGAATTTATACACTATTTTCTGATACCATG(+ATATAT)GTACCATCTTTGTCTTAAAAAAAAAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240606214253_oo18 TATTATAGCTACTGCTGCCGCTACTGTTGTTG(+ATATAT)GTACCACCTCAGATACATAGAGGGACACATA NGG 10 8 17 8 24 0 100 5 10
    240606214252_1uwb TGATTTATGTGTCCCTCTATGTATCTGAGGTG(+ATATAT)GTACCAACAACAGTAGCGGCAGCAGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606214250_gbre CGTCTGCTTCCCTCTCTCCTCGTGCTAAGCTG(+ATATAT)GATCCGTGTCCTTTTCTTTTTAACGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606214248_r0h6 TCACTTGAACTCGTTAAAAAGAAAAGGACACG(+ATATAT)GATCCAGCTTAGCACGAGGAGAGAGGGAAGC NGG 10 8 17 8 24 0 100 5 10