Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214228_ic3l AGACTTAATAAAGGGTTTTACAAGGGTACCTG(+ATATAT)GATCCCAGGGGGAAGTAGTTTATCCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606214224_reu4 CTGTGCCCCAAGGATAAACTACTTCCCCCTGG(+ATATAT)GATCCAGGTACCCTTGTAAAACCCTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240606214222_m4pt TCTCAAAGATGAGGCCCACATGAACCTGCTGG(+ATATAT)GATCCCACGAACACATATTGGACCTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606214221_r3mr TCCCTGTGCTCAGGTCCAATATGTGTTCGTGG(+ATATAT)GATCCCAGCAGGTTCATGTGGGCCTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240606214219_xe95 ACCACATGATAGCTCGCAACCATCTATAATAG(+ATATAT)GATCCAAACACTCTTCTGGTGTGTCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606214217_fpii AGCTGTCTTCAGACACACCAGAAGAGTGTTTG(+ATATAT)GATCCTATTATAGATGGTTGCGAGCTATCAT NGG 10 8 17 8 24 0 100 5 10
    240606214216_irmx GTGGCATGCCCAGCCTTCTCTCTGGCCTATTG(+ATATAT)GTACCCCCCACCCCCAAAAGAAACAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606214213_7uw8 TTAACCTCCCTTGTTTCTTTTGGGGGTGGGGG(+ATATAT)GTACCAATAGGCCAGAGAGAAGGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606214211_5dv7 TGGGGGCTCTTAAGGATTTCCCAGAAAAACAG(+ATATAT)GATCCTGGGATGGGACGTGGTTGCTCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606214209_y33v TTTTTTGCCCGAGCAACCACGTCCCATCCCAG(+ATATAT)GATCCTGTTTTTCTGGGAAATCCTTAAGAGC NGG 10 8 17 8 24 0 100 5 10