Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214113_b48p TCTGTGGTGCAGACCATAAAAGGAAGCCATTG(+ATATAT)GTACCTTCTCAGGTACTTAGCTTTTTGGATA NGG 10 8 17 8 24 0 100 5 10
    240606214111_f689 CCTGATATCCAAAAAGCTAAGTACCTGAGAAG(+ATATAT)GTACCAATGGCTTCCTTTTATGGTCTGCACC NGG 10 8 17 8 24 0 100 5 10
    240606214110_lbe2 ATGCAGGAGCACCCCTTTACCCATTTTAAAGG(+ATATAT)GTACCTTGTACATTTATTTAATCCTACTAAT NGG 10 8 17 8 24 0 100 5 10
    240606214108_za64 TGCACATTAGTAGGATTAAATAAATGTACAAG(+ATATAT)GTACCCTTTAAAATGGGTAAAGGGGTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606214107_3spv ACTCTGTAGTTGGAAGAGAATAATAGTGCTGG(+ATATAT)GTACCCCAGAAAGGTACTGCCTGCATTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606214105_dtce TTATGGTGAAATGCAGGCAGTACCTTTCTGGG(+ATATAT)GTACCCAGCACTATTATTCTCTTCCAACTAC NGG 10 8 17 8 24 0 100 5 10
    240606214103_50za AAGGGCACTTGGCTCAGTTGGAGACACTTTTG(+ATATAT)GTACCTGGTTGGGCAAGTGGGGTGGTACTAA NGG 10 8 17 8 24 0 100 5 10
    240606214101_gtne CGGTATTAGTACCACCCCACTTGCCCAACCAG(+ATATAT)GTACCAAAAGTGTCTCCAACTGAGCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606214059_wzlw AAGTGGATTGAGTTTTTAGCACCTGAGTGTGG(+ATATAT)GATCCATTGTCATTTGTTTTCCCAGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240606214058_8wcc AAGTAAAGGTTCTGGGAAAACAAATGACAATG(+ATATAT)GATCCCACACTCAGGTGCTAAAAACTCAATC NGG 10 8 17 8 24 0 100 5 10