Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214006_arpr TATATATTGAACATGTTTATCTCTTGCTCCTG(+ATATAT)GATCCTGTTTCAGTGAGCGCCCACACTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606214005_edxs CCTCCCCACAGTGTGGGCGCTCACTGAAACAG(+ATATAT)GATCCAGGAGCAAGAGATAAACATGTTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606214003_iftd CTCTTTTCCTTCTCTTATTCATTTGAGTGGTG(+ATATAT)GTACCTTATTCTGTGAGAATAATGCTCATGG NGG 10 8 17 8 24 0 100 5 10
    240606214002_t44r GTTCCCCATGAGCATTATTCTCACAGAATAAG(+ATATAT)GTACCACCACTCAAATGAATAAGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606214000_g94l GTGTTGTTGTTGTAGTTTGTTTCTATGCCGTG(+ATATAT)GTACCGTGGTTATCTCCCATGGAGGACTCCG NGG 10 8 17 8 24 0 100 5 10
    240606213958_bkah CAGCCCGGAGTCCTCCATGGGAGATAACCACG(+ATATAT)GTACCACGGCATAGAAACAAACTACAACAAC NGG 10 8 17 8 24 0 100 5 10
    240606213957_g1tt CGAGGATACCCGAATGTGATGAAGGGCTCAGG(+ATATAT)GTACCAGTGATGGGGCCACTGGAGACATCTC NGG 10 8 17 8 24 0 100 5 10
    240606213955_mne2 CCAAGGAGATGTCTCCAGTGGCCCCATCACTG(+ATATAT)GTACCCTGAGCCCTTCATCACATTCGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606213954_4jyb GTCACAGGGCTGGCATCTGGAACACAGCACAG(+ATATAT)GTACCAAGACAAGGGTAAAATCTTGTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606213953_q36n CTGCTGCTTTACAAGATTTTACCCTTGTCTTG(+ATATAT)GTACCTGTGCTGTGTTCCAGATGCCAGCCCT NGG 10 8 17 8 24 0 100 5 10