Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213856_wwa3 GTAAGATACATAAATATAGAATTAATATAAAG(+ATATAT)GTACCACTATGTTTTACTTATAATAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240606213854_yerq AAATGTGTTTTTATTATAAGTAAAACATAGTG(+ATATAT)GTACCTTTATATTAATTCTATATTTATGTAT NGG 10 8 17 8 24 0 100 5 10
    240606213852_9inh CAACTGAGAGAAGTGGGAAGAGCATCTGGTGG(+ATATAT)GTACCAATTGGCAGAGAAGGGGAGAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240606213850_2vd7 ATGACCAGTCTTCTCCCCTTCTCTGCCAATTG(+ATATAT)GTACCCACCAGATGCTCTTCCCACTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606213849_2ane TCTCTTCCACTCAGAAAGTGGCATGGCTTCAG(+ATATAT)GTACCACAGGGCACATATGTCAATCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240606213847_5bzo AGACCAGGGTTGATTGACATATGTGCCCTGTG(+ATATAT)GTACCTGAAGCCATGCCACTTTCTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606213845_zzzk CCCATGAGAGTGTCGGGGTCAGGGGCTGAAAG(+ATATAT)GATCCAACATTTCCAAGTTAGAAGTTAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606213844_iist GTTAAAGCTTAACTTCTAACTTGGAAATGTTG(+ATATAT)GATCCTTTCAGCCCCTGACCCCGACACTCTC NGG 10 8 17 8 24 0 100 5 10
    240606213842_9a66 TGGCAGGATCAACATGTGTAACTGCCTTCTTG(+ATATAT)GTACCTGTTTGATTAGTGGGTAGAAAATAGC NGG 10 8 17 8 24 0 100 5 10
    240606213841_q3yx TAACTGCTATTTTCTACCCACTAATCAAACAG(+ATATAT)GTACCAAGAAGGCAGTTACACATGTTGATCC NGG 10 8 17 8 24 0 100 5 10