Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213820_y86d GATAGGGAGGGCATGCCTCGCTTTGGATGAGG(+ATATAT)GATCCATTTAAACACTACCGTTTGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240606213818_jeyk AGCCCTCAATTCCAAACGGTAGTGTTTAAATG(+ATATAT)GATCCCTCATCCAAAGCGAGGCATGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606213817_i4n6 ATCCTCTGCCCGCAATGCTTTTGAAAGACTTG(+ATATAT)GATCCTTTTGAAATGGATGCTGAGCTGCACT NGG 10 8 17 8 24 0 100 5 10
    240606213816_a0c7 CGCACAGTGCAGCTCAGCATCCATTTCAAAAG(+ATATAT)GATCCAAGTCTTTCAAAAGCATTGCGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606213814_vngn AGCCAGTGGAACGGAAGGTCCAAGTACCAAAG(+ATATAT)GATCCTCAGAACACTCCCTACAGTCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606213813_x7q9 CGGTGCCAGGAGACTGTAGGGAGTGTTCTGAG(+ATATAT)GATCCTTTGGTACTTGGACCTTCCGTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606213811_fsan TTAAAGCAACCAAAGACAACTTGGGAACTAAG(+ATATAT)GATCCTGGAGCACTACAGCCCCTGCAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606213809_7dz0 CAGGGGACACTGCAGGGGCTGTAGTGCTCCAG(+ATATAT)GATCCTTAGTTCCCAAGTTGTCTTTGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606213808_okwj GCCTATGTGTGCGAGGACTGTGCATCAGAAAG(+ATATAT)GTACCACGGACCACAGAGTTCAAACCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606213805_wpp3 TTGGGCAGAAGGTTTGAACTCTGTGGTCCGTG(+ATATAT)GTACCTTTCTGATGCACAGTCCTCGCACACA NGG 10 8 17 8 24 0 100 5 10