Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213707_whb6 GTCAGGCTTTTCCCTCAGAAGATCACAGTTTG(+ATATAT)GATCCTGAGAGGAATCTGCATGGGCTTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606213706_h0dn GAGAGGCTGAAGCCCATGCAGATTCCTCTCAG(+ATATAT)GATCCAAACTGTGATCTTCTGAGGGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606213705_lz0m GAGGCAACTTATTCCACCTTTTGGGTGCTGTG(+ATATAT)GATCCCCCGGCTCCCCTCAGTCCCCTAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606213702_dw2c AAACAAGCTTAGGGGACTGAGGGGAGCCGGGG(+ATATAT)GATCCACAGCACCCAAAAGGTGGAATAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606213700_5ir9 AAAGGGGTTTTGATAGAAGGAAGAGACTTCCG(+ATATAT)GTACCATTGTAACTAAAGTGTCAGTCATTTT NGG 10 8 17 8 24 0 100 5 10
    240606213659_6cy8 TAGTTAAAATGACTGACACTTTAGTTACAATG(+ATATAT)GTACCGGAAGTCTCTTCCTTCTATCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240606213657_k8we ACATCTCCTGCCTCAGTCTTCAAAGCTATTTG(+ATATAT)GATCCATAAGTTTGTTATATGCATCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606213655_j07o ACATGCAAGAAGATGCATATAACAAACTTATG(+ATATAT)GATCCAAATAGCTTTGAAGACTGAGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606213654_0fc2 CAAGGTAATGAGTTTCGTGGTAGCATTTTGAG(+ATATAT)GATCCATGTCATCGTTCATTGTTCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606213653_jeq0 GGGCTAGGAAAAGAACAATGAACGATGACATG(+ATATAT)GATCCTCAAAATGCTACCACGAAACTCATTA NGG 10 8 17 8 24 0 100 5 10