Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213725_yk6f TAACGTTGACACAAACACAGATAAGCTCGTGG(+ATATAT)GTACCTGGACAAAAGGGTGCCTTAGTTTATG NGG 10 8 17 8 24 0 100 5 10
    240606213723_bi2o GAGGACATAAACTAAGGCACCCTTTTGTCCAG(+ATATAT)GTACCCACGAGCTTATCTGTGTTTGTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606213721_a3at GTGACAAGTTAGAATGTCTCTCTTGGTTTGTG(+ATATAT)GTACCCCCAGGTTGGCTTTGTTCTGTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606213719_9yc0 GGACTTTTCCACAGAACAAAGCCAACCTGGGG(+ATATAT)GTACCACAAACCAAGAGAGACATTCTAACTT NGG 10 8 17 8 24 0 100 5 10
    240606213717_z51j TCTGCTGGCATACCACATGACACAAAGCAGAG(+ATATAT)GATCCCAGCAAACTAGTGGGATCTCGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606213716_eqvk GGGCCCATTCCGAGATCCCACTAGTTTGCTGG(+ATATAT)GATCCTCTGCTTTGTGTCATGTGGTATGCCA NGG 10 8 17 8 24 0 100 5 10
    240606213714_aqr1 AGTGGCACAGACGCCCAGCGCCAGGAGTATTG(+ATATAT)GTACCACCTCTCCTGTTTTAACTGGAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606213713_ek6m TCCATCCCCTTCCAGTTAAAACAGGAGAGGTG(+ATATAT)GTACCAATACTCCTGGCGCTGGGCGTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606213710_9m8a GGTGTGTTTCCAGGAGCCAGCACCCCACCAGG(+ATATAT)GTACCGCATAAACATTGCAGGAGAGTTTCCG NGG 10 8 17 8 24 0 100 5 10
    240606213709_4wox GGCTGCGGAAACTCTCCTGCAATGTTTATGCG(+ATATAT)GTACCCTGGTGGGGTGCTGGCTCCTGGAAAC NGG 10 8 17 8 24 0 100 5 10