Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214834_ubxy AATATGAGTCTGAATGGCTTATTCACAAATGG(+ATATAT)GATCCGGATTCAGTGGTTGGAATGCAGACAC NGG 10 8 17 8 24 0 100 5 10
    240606214832_bm3a GGAAACTGAAGTGTGTGCACACTTGAAGGAAG(+ATATAT)GATCCCGAGTTTTCATATATTTTTAATATAT NGG 10 8 17 8 24 0 100 5 10
    240606214830_uw0v TCTTAATATATTAAAAATATATGAAAACTCGG(+ATATAT)GATCCTTCCTTCAAGTGTGCACACACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606214829_4lqd ACAGACCAGGTAAAGAGAAAGCCCTAGTACAG(+ATATAT)GTACCTTGCAATCAACCATGCTTGGTTCAAC NGG 10 8 17 8 24 0 100 5 10
    240606214827_ogwx TCAAGGTTGAACCAAGCATGGTTGATTGCAAG(+ATATAT)GTACCTGTACTAGGGCTTTCTCTTTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240606214825_7ufj CAGAGCCAACAGCTTCTCATTCTAAAGCAAGG(+ATATAT)GATCCCTAGAGTTTGGAGCTTCATGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606214823_wht0 CCTAACCCCAACATGAAGCTCCAAACTCTAGG(+ATATAT)GATCCCTTGCTTTAGAATGAGAAGCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606214820_lis9 GCCTCTGTGTAGTCTGGATCTACTGACACTGG(+ATATAT)GTACCAGGGTTTCCTTCTGTTGACTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240606214817_y8lk GCTGCAATCACAGTCAACAGAAGGAAACCCTG(+ATATAT)GTACCCAGTGTCAGTAGATCCAGACTACACA NGG 10 8 17 8 24 0 100 5 10
    240606214816_50dw GTGGAATTTCTCCAGGGTGGAATTTTAGACTG(+ATATAT)GTACCAGAAGTCGGCAAGGCGTGACTGCTGG NGG 10 8 17 8 24 0 100 5 10