Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213932_m7z7 GAACTAGGTTAGATACAAATGGGCAAATACTG(+ATATAT)GTACCAGGGTCTGGAGAAGGGCAGGAAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606213930_0fek TAAAACACTTTCCTGCCCTTCTCCAGACCCTG(+ATATAT)GTACCAGTATTTGCCCATTTGTATCTAACCT NGG 10 8 17 8 24 0 100 5 10
    240606213928_35lt CCTTGTCACTCAGTTTTGATCCATGGAGTCGG(+ATATAT)GATCCCAGTTTTTAAGCAGGCAAGAGGAACC NGG 10 8 17 8 24 0 100 5 10
    240606213926_6zvg ATCCTGGTTCCTCTTGCCTGCTTAAAAACTGG(+ATATAT)GATCCCGACTCCATGGATCAAAACTGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606213925_fcvd CCAGCACTTTTGTTCACTTAAAATGTTGGAAG(+ATATAT)GATCCAGGCCCTTTTCAGGAAGAAGCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606213923_lwv7 TCTGGGGGCAGCTTCTTCCTGAAAAGGGCCTG(+ATATAT)GATCCTTCCAACATTTTAAGTGAACAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606213921_delp AAAAAAAAAACAGAAACAAAAAATGGTGCCAG(+ATATAT)GTACCACAAGAGTAACAGAACTGTGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606213920_y87d CCAGAAAACAGCACAGTTCTGTTACTCTTGTG(+ATATAT)GTACCTGGCACCATTTTTTGTTTCTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606213918_ssd0 AGGCCACTTCTCCAAGACAGTTTCTGACACTG(+ATATAT)GTACCTTCCCCTAATCATGTATCTCCCAAAA NGG 10 8 17 8 24 0 100 5 10
    240606213917_g9vo TCATCTTTTGGGAGATACATGATTAGGGGAAG(+ATATAT)GTACCAGTGTCAGAAACTGTCTTGGAGAAGT NGG 10 8 17 8 24 0 100 5 10