Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214024_hgfc TTATACCACTAAGAAACCATGTACATAAATGG(+ATATAT)GATCCAAATATAGTTTGGAGACAACCCAACT NGG 10 8 17 8 24 0 100 5 10
    240606214022_84eg ATATTAGTTGGGTTGTCTCCAAACTATATTTG(+ATATAT)GATCCCATTTATGTACATGGTTTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606214020_ysa0 AGAAACAGATTTGTATTGTGTTTTATGGCCTG(+ATATAT)GATCCAGGAGTACTTTTATAATGGGTAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606214018_39n5 TGTTCATCTTACCCATTATAAAAGTACTCCTG(+ATATAT)GATCCAGGCCATAAAACACAATACAAATCTG NGG 10 8 17 8 24 0 100 5 10
    240606214017_qwvt ACTAAGATTTGAAATCAGCCTTCTTAAAGGAG(+ATATAT)GATCCCTTGCCAAAGCAGAGATCAGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606214015_1c7w TGGAAGCTTTGCTGATCTCTGCTTTGGCAAGG(+ATATAT)GATCCTCCTTTAAGAAGGCTGATTTCAAATC NGG 10 8 17 8 24 0 100 5 10
    240606214013_zcdx GTTAGCAGTCACAGGATTAAGAAAGATTTGGG(+ATATAT)GATCCAAGACTAGTGAGTTCTAAGGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606214011_oqqp GGCCTGCCTGTCCTTAGAACTCACTAGTCTTG(+ATATAT)GATCCCCAAATCTTTCTTAATCCTGTGACTG NGG 10 8 17 8 24 0 100 5 10
    240606214009_lh49 CTCTTTTCCTTCTCTTATTCATTTGAGTGGTG(+ATATAT)GTACCTTATTCTGTGAGAATAATGCTCATGG NGG 10 8 17 8 24 0 100 5 10
    240606214008_7zz1 GTTCCCCATGAGCATTATTCTCACAGAATAAG(+ATATAT)GTACCACCACTCAAATGAATAAGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10