Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215218_l2ud CCCTGCTGCGGAAGCTGCACATGCACTCCCAG(+ATATAT)GATCCTGTGTCTGTGCCCATCCCAGAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606215216_qdst GAGGACAGGGTCCTGCACCCAGTTGGGTCTGG(+ATATAT)GTACCTTGGGGAACACTGGACTTCCAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606215214_iv6j CTCTGGCCTTTGGAAGTCCAGTGTTCCCCAAG(+ATATAT)GTACCCAGACCCAACTGGGTGCAGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606215212_3q58 CCCAACCATGCAGGCTTACTCTCTGTGCTCTG(+ATATAT)GTACCCGAGAAGGGGCCAAGTCTGTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606215210_9h3j ACCCACCCTACACAGACTTGGCCCCTTCTCGG(+ATATAT)GTACCAGAGCACAGAGAGTAAGCCTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240606215209_52mc TAGCTAGCTGGATGCTGTTTACTGGGTGAAAG(+ATATAT)GTACCTTGGGAGTTTTCATGGCTCCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606215207_m3lj CATCCCTGCTTGGAGCCATGAAAACTCCCAAG(+ATATAT)GTACCTTTCACCCAGTAAACAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606215205_j2ac TGGCCCTACAGAACACACCTGAGCCTCTTGGG(+ATATAT)GTACCATCGCAGGGTAGCCCTCCCTAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606215203_esbn AACTAGGCCCTAGGGAGGGCTACCCTGCGATG(+ATATAT)GTACCCCAAGAGGCTCAGGTGTGTTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606215202_vpqx CCAAGAACCAACTAGCTGCCTCAGGTCTCGTG(+ATATAT)GATCCCAGATGGACATGGCAGTAACTCAGAG NGG 10 8 17 8 24 0 100 5 10