Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214130_zhgd GCATAGCCTTCTTCTGTGGGTTACCTCCTCAG(+ATATAT)GTACCCAGGTCACATCCTGGTTAAGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606214128_9qiw CAAGGTGAGAACTTAACCAGGATGTGACCTGG(+ATATAT)GTACCTGAGGAGGTAACCCACAGAAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606214127_thhi TTGAACTAGGCTCTGTCCGTGAAAGTGTGACG(+ATATAT)GATCCTAATTTCAAGGGGACCTAAAACTTCA NGG 10 8 17 8 24 0 100 5 10
    240606214125_o1r4 TTCCTTGAAGTTTTAGGTCCCCTTGAAATTAG(+ATATAT)GATCCGTCACACTTTCACGGACAGAGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240606214124_pzgq TAAAGCCCCCTACTGCACCCCTGACCTGTGAG(+ATATAT)GATCCATTTTTTTTTCTTTCCTTCCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606214122_fgsw CCGAGATTTGGGGAAGGAAAGAAAAAAAAATG(+ATATAT)GATCCTCACAGGTCAGGGGTGCAGTAGGGGG NGG 10 8 17 8 24 0 100 5 10
    240606214120_w43l CAGAAGTTAAGGTGGCCACAGCACAGGTCAGG(+ATATAT)GATCCAAGGAGCTGGGGAGGACTGCTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240606214118_16p8 ACTAGTTTTGAGCAGTCCTCCCCAGCTCCTTG(+ATATAT)GATCCCTGACCTGTGCTGTGGCCACCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606214116_dj85 TCTAACTCCATTGGGGACAGGCTAGGGGATGG(+ATATAT)GATCCTCTACCTTTTTCAGGCCTCTGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606214115_c86d CTGGTGTCTGCAGAGGCCTGAAAAAGGTAGAG(+ATATAT)GATCCCATCCCCTAGCCTGTCCCCAATGGAG NGG 10 8 17 8 24 0 100 5 10