Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214557_8sab CGACTCAAAGGGCCTTCTTGCTGGGGCCTCTG(+ATATAT)GATCCTCTGTCCTGGGGCCGGAAGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606214555_lo0c TCCGGCCCCTGCCTTCCGGCCCCAGGACAGAG(+ATATAT)GATCCAGAGGCCCCAGCAAGAAGGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606214554_yiq4 TTGTCATTCATCACGAAGTAGGCCAAATCATG(+ATATAT)GTACCACAGGAATTTAGAAATCTAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240606214552_pm36 CCAGATTGCAGTTAGATTTCTAAATTCCTGTG(+ATATAT)GTACCATGATTTGGCCTACTTCGTGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240606214551_v1gq AAAGCCCTGGGTTGACTGACTCCCTCACTCAG(+ATATAT)GATCCTAAGAACAATGCTGACTGCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606214550_529n AAGTAGAAAACTGCAGTCAGCATTGTTCTTAG(+ATATAT)GATCCTGAGTGAGGGAGTCAGTCAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606214548_n73h GTGCAAGCGCCATGGCACCCTGCTGTACCACG(+ATATAT)GTACCAAACTCAACTGTACAGTCAACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606214546_ac0b CTCGCTGACTGTTGACTGTACAGTTGAGTTTG(+ATATAT)GTACCGTGGTACAGCAGGGTGCCATGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240606214545_knf6 CGTAGGCCGATGAGTGTAAGTAAAAGAGTGTG(+ATATAT)GTACCACTGGAAAAAACTCCAAACCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606214543_iwey TCGATGGCCCTGGTTTGGAGTTTTTTCCAGTG(+ATATAT)GTACCACACTCTTTTACTTACACTCATCGGC NGG 10 8 17 8 24 0 100 5 10