Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214706_oevu GTTGTTTGGTTTGTTTATTTCTGTGGTTCTGG(+ATATAT)GATCCAGCCCAGGGCCTTACATGTGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606214704_f50h TATTTGTCTAGCACATGTAAGGCCCTGGGCTG(+ATATAT)GATCCCAGAACCACAGAAATAAACAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240606214702_kezk GTTACAGCAGGGATTTGACAAAACACAGGGTG(+ATATAT)GATCCATGCAGTTTGGAAACTTAACTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606214701_wo3q GATCGTTCAAAGTTAAGTTTCCAAACTGCATG(+ATATAT)GATCCACCCTGTGTTTTGTCAAATCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606214659_0voo TTTCTAAGCCACACAAGATGGGGTAAAAAGGG(+ATATAT)GTACCAACACCTCAATGGCAGTTAGACTTCC NGG 10 8 17 8 24 0 100 5 10
    240606214657_eceq TGAGTGGAAGTCTAACTGCCATTGAGGTGTTG(+ATATAT)GTACCCCTTTTTACCCCATCTTGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606214655_4kgy ATGTGAATCCAGAGCTTGCTGGGGAGCCAGAG(+ATATAT)GTACCATTCAATGGCAGAGCTCTTCCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240606214654_jiry CTTATGCTAGGGAAGAGCTCTGCCATTGAATG(+ATATAT)GTACCTCTGGCTCCCCAGCAAGCTCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240606214652_155k TCCGATGTCTGATGTGTCATCTTTTTATCTTG(+ATATAT)GATCCTTCCCTGGTTTGAAACTTTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240606214650_m6bw TTTCAAGAGTTTAAAGTTTCAAACCAGGGAAG(+ATATAT)GATCCAAGATAAAAAGATGACACATCAGACA NGG 10 8 17 8 24 0 100 5 10