Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213507_p7v2 TGCACACAGTGAGGAATAAACATTCTGGAAAG(+ATATAT)GATCCTTGATTCTCCCACAAACTGTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606213505_p80n TACTCCAAGGAACAGTTTGTGGGAGAATCAAG(+ATATAT)GATCCTTTCCAGAATGTTTATTCCTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240606213504_wg91 TTTCCTTTTTAAATCATCCTGTGTGCATTCAG(+ATATAT)GATCCTGGTCTTCATACAGAGATGTTGACAC NGG 10 8 17 8 24 0 100 5 10
    240606213502_8voh GAAGGGTGTCAACATCTCTGTATGAAGACCAG(+ATATAT)GATCCTGAATGCACACAGGATGATTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606213459_7vt0 TTTGGTCTGGACTATGGGCTCATCAGGCCCAG(+ATATAT)GATCCCGGACCAAAGACTGAGATGACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606213457_jv67 TATTGGAAGGGTCATCTCAGTCTTTGGTCCGG(+ATATAT)GATCCTGGGCCTGATGAGCCCATAGTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606213455_z786 ATGCATGCTCTCTGTCTTTTGCTACAAAAATG(+ATATAT)GTACCCAGATCTGCCTACTGCTTTCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606213454_malg GGGGAGGGCAGGAAAGCAGTAGGCAGATCTGG(+ATATAT)GTACCATTTTTGTAGCAAAAGACAGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606213453_czf8 ATGCATGCTCTCTGTCTTTTGCTACAAAAATG(+ATATAT)GTACCCAGATCTGCCTACTGCTTTCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606213451_zerc GGGGAGGGCAGGAAAGCAGTAGGCAGATCTGG(+ATATAT)GTACCATTTTTGTAGCAAAAGACAGAGAGCA NGG 10 8 17 8 24 0 100 5 10