Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213358_hqo7 CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+ATATAT)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606213355_76y6 TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+ATATAT)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606213353_17wf CTTCATTGGCATGGCAACAGGTAGGCATATGG(+ATATAT)GATCCTCCTAGTGGTTAGTACAATCACACCA NGG 10 8 17 8 24 0 100 5 10
    240606213352_dpze CACAGTGGTGTGATTGTACTAACCACTAGGAG(+ATATAT)GATCCCATATGCCTACCTGTTGCCATGCCAA NGG 10 8 17 8 24 0 100 5 10
    240606213350_vdf1 ACATGGACCTTTGGAAACCTGTGGATGACATG(+ATATAT)GATCCGTGAAGAGCCCGGGTCACTACCACTC NGG 10 8 17 8 24 0 100 5 10
    240606213349_x92t GAAATGAGTGGTAGTGACCCGGGCTCTTCACG(+ATATAT)GATCCATGTCATCCACAGGTTTCCAAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606213348_1fha TCAAATCTATACACAGTAGGGCTATGAAGTTG(+ATATAT)GTACCTCAGAAGTTCCTCCCCTGCCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606213346_ybxy AGGTCCCAACAGGCAGGGGAGGAACTTCTGAG(+ATATAT)GTACCAACTTCATAGCCCTACTGTGTATAGA NGG 10 8 17 8 24 0 100 5 10
    240606213344_zrc4 GCTCCGAGGTCAGGTGGACTGTCAGGGCCTAG(+ATATAT)GATCCACCAGTTTGCCCGAGTTTACATTAGC NGG 10 8 17 8 24 0 100 5 10
    240606213342_i399 ATGTTGCTAATGTAAACTCGGGCAAACTGGTG(+ATATAT)GATCCTAGGCCCTGACAGTCCACCTGACCTC NGG 10 8 17 8 24 0 100 5 10