Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213339_29fm CTCCTGAGCCAATAGGGGCTTCCAGACCCAAG(+ATATAT)GATCCGTTTCCTTGATGACTAAGAAGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606213338_03cj AAGGGTGGGGCTTCTTAGTCATCAAGGAAACG(+ATATAT)GATCCTTGGGTCTGGAAGCCCCTATTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606213336_mcmb TCCAAGAGCCCTGCTCTGCCCCTCGTGGCATG(+ATATAT)GATCCTTTCCCTGGTGTGGACTTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606213335_40f4 TCCCCAGAGACTTAAGTCCACACCAGGGAAAG(+ATATAT)GATCCATGCCACGAGGGGCAGAGCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606213333_iqok CAGGAGGCTGCTATGGGCTAAAATTTTCCTAG(+ATATAT)GTACCACCTAGAGGAGATGGAGCCGACCGAG NGG 10 8 17 8 24 0 100 5 10
    240606213331_5l7r CTTCCCTCGGTCGGCTCCATCTCCTCTAGGTG(+ATATAT)GTACCTAGGAAAATTTTAGCCCATAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606213329_6vne CAGGAATGCCATCTTCTGCCTTGGTCTGGAAG(+ATATAT)GTACCCATGGCTACTTGCATCATTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606213327_450o ACAAGGCTGGAAAATGATGCAAGTAGCCATGG(+ATATAT)GTACCTTCCAGACCAAGGCAGAAGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606213325_me4z AAAAAGACCTGTGGAGATCTCAGAATCCACCG(+ATATAT)GTACCATACTGCAAGGGATACTGACCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240606213323_cz1u AGGAACTTAAGGTCAGTATCCCTTGCAGTATG(+ATATAT)GTACCGGTGGATTCTGAGATCTCCACAGGTC NGG 10 8 17 8 24 0 100 5 10