Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213229_htf4 CCTTGGTTCTGTTGCTTACTGGCCGAGAAACG(+ATATAT)GTACCAATCTTTCAGCTCTGTGCTTGTCGGC NGG 10 8 17 8 24 0 100 5 10
    240606213228_5tp0 AAGAAGCCGACAAGCACAGAGCTGAAAGATTG(+ATATAT)GTACCGTTTCTCGGCCAGTAAGCAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606213226_qjxb TCGTCAGCAGCCTGTCTCATTGCTTCAGCTGG(+ATATAT)GTACCAGTCCTTCTGTTCCCCTGGGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240606213224_278a GAGTTCAGTTTCCCAGGGGAACAGAAGGACTG(+ATATAT)GTACCCAGCTGAAGCAATGAGACAGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606213222_muzy TGCAATCCCAGGGCTGGGAGATGGAGCCAGAG(+ATATAT)GATCCCTGGGACTTGCTGGCCAGCCAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606213219_32x9 TTAACTAGCCTGGCTGGCCAGCAAGTCCCAGG(+ATATAT)GATCCTCTGGCTCCATCTCCCAGCCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606213217_y0ze GTGGTATTGCATTTAAATGGACAGGACACGGG(+ATATAT)GTACCAAATCCTACAATGTGTCAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606213216_u22z AATTGCTCCAAACTGACACATTGTAGGATTTG(+ATATAT)GTACCCCGTGTCCTGTCCATTTAAATGCAAT NGG 10 8 17 8 24 0 100 5 10
    240606213214_dtls TGCTCTTTATTCTCAGGGCCCGCACACATCCG(+ATATAT)GTACCTTCATAAATGCAGTGTCACTGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606213212_k3fu TCCATTTCTTCAGTGACACTGCATTTATGAAG(+ATATAT)GTACCGGATGTGTGCGGGCCCTGAGAATAAA NGG 10 8 17 8 24 0 100 5 10