Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606214423_5ob2 ACTACACGGTGACTCACAACCATCTGTAATGG(+ATATAT)GATCCGATGCTCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606214422_nxz9 TAGTCCTCAGTCAGGCTAATACAGGAGTCAGG(+ATATAT)GTACCAGCTCCCTTATACTTTGTTTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606214420_ljfb TACCACCTTCAAAACAAAGTATAAGGGAGCTG(+ATATAT)GTACCCTGACTCCTGTATTAGCCTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240606214417_7oki TGATAACACACAAGTGTACAGCACCCGCCGGG(+ATATAT)GATCCCAAAGCCTGCTGGTGCTCAGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606214415_s5fx TCCAACTGCCCCTGAGCACCAGCAGGCTTTGG(+ATATAT)GATCCCCGGCGGGTGCTGTACACTTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606214414_tkwk GGTTAGACATGTTTTAAAAACTCCTTTATGTG(+ATATAT)GTACCATTGTCTGTTTACTTAATGCTCATGA NGG 10 8 17 8 24 0 100 5 10
    240606214413_7j02 GTTGCTCATGAGCATTAAGTAAACAGACAATG(+ATATAT)GTACCACATAAAGGAGTTTTTAAAACATGTC NGG 10 8 17 8 24 0 100 5 10
    240606214411_wiik GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+ATATAT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606214408_167w GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+ATATAT)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606214407_yqke CTTTGGGGACCAACCTTGTTGCGTAGTGTGTG(+ATATAT)GTACCAGCTTTGCGCTTTGCCTGGGAAAACT NGG 10 8 17 8 24 0 100 5 10