Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213210_b25w AGCACACTGCTATGTGTGGCCTTGATGCTGGG(+ATATAT)GATCCTAACTCAGGTTCTCGTGCTTTACCGA NGG 10 8 17 8 24 0 100 5 10
    240606213209_mama CCCAGTCGGTAAAGCACGAGAACCTGAGTTAG(+ATATAT)GATCCCCAGCATCAAGGCCACACATAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606213207_c990 AGAAACCAGGGCCAGCGTTGGGGGTGCAGGAG(+ATATAT)GTACCCAGAGAAGCAAAAACATTCAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606213206_xmim CTCTTTTCCTTTGAATGTTTTTGCTTCTCTGG(+ATATAT)GTACCTCCTGCACCCCCAACGCTGGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606213203_lrrl TAAAATTCAGTATTTAAATATTAATTGCCAGG(+ATATAT)GTACCAAATAAATTGTCACTTATAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240606213202_68x9 ACAAGAAATATTTATAAGTGACAATTTATTTG(+ATATAT)GTACCCTGGCAATTAATATTTAAATACTGAA NGG 10 8 17 8 24 0 100 5 10
    240606213200_6d7h TGTGAAAACAGCCCTTTTAGAGTATTTATTAG(+ATATAT)GATCCTAAGCTAATCGTAGCCTTCACAAATT NGG 10 8 17 8 24 0 100 5 10
    240606213159_f49i GTAACAATTTGTGAAGGCTACGATTAGCTTAG(+ATATAT)GATCCTAATAAATACTCTAAAAGGGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606213157_o4t3 GGATTGCCCCACACCGTCCCCAGTCAGCCCTG(+ATATAT)GATCCATGTGTCCTTTACTTGGGGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240606213155_gslm GTAGAAGGTTTTCCCCAAGTAAAGGACACATG(+ATATAT)GATCCAGGGCTGACTGGGGACGGTGTGGGGC NGG 10 8 17 8 24 0 100 5 10