Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213102_45lt CAACTTAGGGGGTGAGCAGGTGTTGGTGATGG(+ATATAT)GATCCCTGTGATGAGATGGGGGTGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240606213100_ifkj AATGTGCAGTGTCACCCCCATCTCATCACAGG(+ATATAT)GATCCCATCACCAACACCTGCTCACCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240606213059_rcij TCGAATATTTAGAAGTGTAAAACATAAACCAG(+ATATAT)GATCCTGCCCCCCACATGTCCAGGACGATGA NGG 10 8 17 8 24 0 100 5 10
    240606213057_0fp3 AACACTCATCGTCCTGGACATGTGGGGGGCAG(+ATATAT)GATCCTGGTTTATGTTTTACACTTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240606213056_0gzx TAAATTTCACCCTCTGCATTACTCACAGAGTG(+ATATAT)GATCCATTGGAGTTTCAGCTGTATGCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606213054_qvs2 AGCTGAACTAGCATACAGCTGAAACTCCAATG(+ATATAT)GATCCACTCTGTGAGTAATGCAGAGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606213052_fokw GCCAACATTCCTAAGACTGAACCAACATCCAG(+ATATAT)GTACCCATTTTCTCAGGTTTCCCAGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240606213050_bnxx GCCACATATCTCTGGGAAACCTGAGAAAATGG(+ATATAT)GTACCTGGATGTTGGTTCAGTCTTAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606213049_m1ui AGAGAAGAACTCTTATAGACTTGGGGAGAGAG(+ATATAT)GTACCAACCTTGACATTGACTGTACTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606213048_c0q6 AATACTCACCAGTACAGTCAATGTCAAGGTTG(+ATATAT)GTACCTCTCTCCCCAAGTCTATAAGAGTTCT NGG 10 8 17 8 24 0 100 5 10