Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213030_zs8z TGGGTATGGATGGAGCCACACTCCTATGCAAG(+ATATAT)GATCCATTTCCATGCAAGATTGTCAATTCTT NGG 10 8 17 8 24 0 100 5 10
    240606213029_uv3w TCTGGAAGAATTGACAATCTTGCATGGAAATG(+ATATAT)GATCCTTGCATAGGAGTGTGGCTCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240606213027_ql7y TTTTTAAGATGCTTCTGGAGATTTAGAATCAG(+ATATAT)GATCCAAAATAAGGCTGGGATTCAGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606213026_o6fz AGTGGTGGATGCTGAATCCCAGCCTTATTTTG(+ATATAT)GATCCTGATTCTAAATCTCCAGAAGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240606213023_9czu TGAGCGTATCTGCTGTCCTTCTGCATGCCCAG(+ATATAT)GTACCTCAGTTTTTTTCAACAGCAAGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606213022_3gmq CCCTAGTTTCCTTGCTGTTGAAAAAAACTGAG(+ATATAT)GTACCTGGGCATGCAGAAGGACAGCAGATAC NGG 10 8 17 8 24 0 100 5 10
    240606213021_f4lm TTCCTTTGGTTTAAGCCCGTTATAATTAAATG(+ATATAT)GTACCAAAAGCTTTAAGAAGTGCTGTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240606213019_27tj CACAAGTAGAACAGCACTTCTTAAAGCTTTTG(+ATATAT)GTACCATTTAATTATAACGGGCTTAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240606213018_u6n7 AAGAAGTAGGCACATGGAGCAGGTCCTAGGAG(+ATATAT)GTACCCCCCTGCTCAAGTCAAGTTCTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606213015_nit6 TAGAGGGGACAGAACTTGACTTGAGCAGGGGG(+ATATAT)GTACCTCCTAGGACCTGCTCCATGTGCCTAC NGG 10 8 17 8 24 0 100 5 10