Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213046_24vu TGGAACCCAGTTACAGAAATTGTGTGATATAG(+ATATAT)GTACCTTTTTCTCTTTCTCTGTCCAAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606213044_rht0 AAAGCATGTCTTGGACAGAGAAAGAGAAAAAG(+ATATAT)GTACCTATATCACACAATTTCTGTAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240606213043_6tet CCATTTCAAGGTGCAGTGTGAAGTATGACTAG(+ATATAT)GTACCTCTCCATTTATTGAGGCTGGAACAGG NGG 10 8 17 8 24 0 100 5 10
    240606213041_1f3g GATTTCCTGTTCCAGCCTCAATAAATGGAGAG(+ATATAT)GTACCTAGTCATACTTCACACTGCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240606213040_39i5 CTTCAGCTGGAGTGTGCTCTGCTGGTGCAGTG(+ATATAT)GATCCTAGTGTGGTTCCCAGTACTCAACCTG NGG 10 8 17 8 24 0 100 5 10
    240606213038_nak1 CCAACCAGGTTGAGTACTGGGAACCACACTAG(+ATATAT)GATCCACTGCACCAGCAGAGCACACTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606213036_6c3p AGAGACAAAGGACACAGGAACCCGGCTGACTG(+ATATAT)GTACCAAAGTGCACTCTCTTCAGGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606213034_1tex TGTGTAGAGGCACCTGAAGAGAGTGCACTTTG(+ATATAT)GTACCAGTCAGCCGGGTTCCTGTGTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606213032_p62h TGTCTTTATTCTAATGTCTTTGGTGTGGGAAG(+ATATAT)GTACCTCATAGGCTGCCAAAAGAGAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240606213031_ub1z ATGTTTGCATTTCTCTTTTGGCAGCCTATGAG(+ATATAT)GTACCTTCCCACACCAAAGACATTAGAATAA NGG 10 8 17 8 24 0 100 5 10