Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212902_36ne CCACCAAGTGCACAGCACCCACCCCGTATGTG(+ATATAT)GTACCCTGTACGCGTGGATCTTTGTGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240606212900_3434 GATCTGTGCACACAAAGATCCACGCGTACAGG(+ATATAT)GTACCACATACGGGGTGGGTGCTGTGCACTT NGG 10 8 17 8 24 0 100 5 10
    240606212859_gau0 CCTCTGGGACGTCTGTGATGCTCCTTCTCTGG(+ATATAT)GTACCTGAAGAGGCTCCTCCACGACCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606212857_yz9p GAGATGGCTTGGTCGTGGAGGAGCCTCTTCAG(+ATATAT)GTACCCAGAGAAGGAGCATCACAGACGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606212856_l4m5 AAGCTCACCCCATCCCTGTGCGGTTGCCTGAG(+ATATAT)GTACCTTCACGAGGAGAGATACACTGGCCCG NGG 10 8 17 8 24 0 100 5 10
    240606212854_k801 GCGGACGGGCCAGTGTATCTCTCCTCGTGAAG(+ATATAT)GTACCTCAGGCAACCGCACAGGGATGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606212853_56gk CAGCAACATCTTCTTCTCAGGGCCACACACTG(+ATATAT)GATCCTGGGAGAGATGCCCACTTAAGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606212851_n7v4 ACCCTGGAAGCTTAAGTGGGCATCTCTCCCAG(+ATATAT)GATCCAGTGTGTGGCCCTGAGAAGAAGATGT NGG 10 8 17 8 24 0 100 5 10
    240606212849_braf CAAGGACAGATGTGACCTGATGATGAAAGAGG(+ATATAT)GATCCCTCAAGGGCCCAGAAACCTGGGGACA NGG 10 8 17 8 24 0 100 5 10
    240606212847_2v65 TCTCGTGTCCCCAGGTTTCTGGGCCCTTGAGG(+ATATAT)GATCCCTCTTTCATCATCAGGTCACATCTGT NGG 10 8 17 8 24 0 100 5 10