Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212751_wy2o GAGACGAGGGGCGGGAGGTCAGCTTCTCCCTG(+ATATAT)GATCCTCAGTGTGCACTGCCTTGGGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606212750_mfjs GTGGCTCCCTGCCCAAGGCAGTGCACACTGAG(+ATATAT)GATCCAGGGAGAAGCTGACCTCCCGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606212748_gbcg GACCCGCGGCTTGCGCATCTGTGACATTTCTG(+ATATAT)GATCCCGGTCAGTTCCGGCTGTGCCCAGACT NGG 10 8 17 8 24 0 100 5 10
    240606212746_0whe TCTCCAGTCTGGGCACAGCCGGAACTGACCGG(+ATATAT)GATCCAGAAATGTCACAGATGCGCAAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240606212745_530g GTGGTTGCAACTTCTGGAGCAGGTGGGTGTGG(+ATATAT)GATCCCAGGTGTAGTGTTGGCTGAGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606212742_c89q TGTCATCTGAGCTCAGCCAACACTACACCTGG(+ATATAT)GATCCCACACCCACCTGCTCCAGAAGTTGCA NGG 10 8 17 8 24 0 100 5 10
    240606212741_hqtn AGAGTCCCAACTCCAGTGAGCAGTAGGGCAGG(+ATATAT)GATCCCACTCAGATCACAAAGTCATCATGGT NGG 10 8 17 8 24 0 100 5 10
    240606212739_floy CGCGGACCATGATGACTTTGTGATCTGAGTGG(+ATATAT)GATCCCTGCCCTACTGCTCACTGGAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606212736_hkvx TGGCAGTCATAGTGCCTAGAGCTTCTTCCCTG(+ATATAT)GTACCCAGGAGTTCCTCACCACTCCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606212733_ofbn TAACTTCCAGGGGAGTGGTGAGGAACTCCTGG(+ATATAT)GTACCAGGGAAGAAGCTCTAGGCACTATGAC NGG 10 8 17 8 24 0 100 5 10