Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212846_6b2k TGAGAATGCCATGTTTTAGGGACCAGCACCTG(+ATATAT)GATCCCTGACACTGTAGTCTCATGGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606212843_1zix GTGCCCAGGGCCCATGAGACTACAGTGTCAGG(+ATATAT)GATCCAGGTGCTGGTCCCTAAAACATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606212841_wuiw GCTCCCTACACGCAGAGTCACTGGTTCAAGGG(+ATATAT)GATCCTAGCTGGGATGTATCTCAAGGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606212840_u6b3 GGTATTCTACCCTTGAGATACATCCCAGCTAG(+ATATAT)GATCCCCTTGAACCAGTGACTCTGCGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606212839_v0b3 AACACGGAACTCCCAGGATTCTGGAATGACAG(+ATATAT)GATCCTCCCAGAAGGTGTGTATACCCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606212837_u9m0 TGTGTTTAAGGGGTATACACACCTTCTGGGAG(+ATATAT)GATCCTGTCATTCCAGAATCCTGGGAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606212836_vqen GGGAAAGTCACATGCTAGCCTCTCCAGCTTCG(+ATATAT)GATCCAGGGCTCCCTTTGATGTGACAACCAT NGG 10 8 17 8 24 0 100 5 10
    240606212833_bcia TACCAATGGTTGTCACATCAAAGGGAGCCCTG(+ATATAT)GATCCGAAGCTGGAGAGGCTAGCATGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606212832_7kal GTGCCCGGTGTGCAGGCACCAAGGAGACACAG(+ATATAT)GTACCAGCAGACCACCACTGGTGACATCATC NGG 10 8 17 8 24 0 100 5 10
    240606212831_nklu CTGGTGATGATGTCACCAGTGGTGGTCTGCTG(+ATATAT)GTACCTGTGTCTCCTTGGTGCCTGCACACCG NGG 10 8 17 8 24 0 100 5 10