Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212732_4rwd AGCTGTCTGCCCTTCATGCTCCAGGGCAGCTG(+ATATAT)GTACCGTGGAAAGTGCCTGGGGTGGTTTTGC NGG 10 8 17 8 24 0 100 5 10
    240606212730_af12 TATTTGCAAAACCACCCCAGGCACTTTCCACG(+ATATAT)GTACCAGCTGCCCTGGAGCATGAAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606212729_gox6 TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+ATATAT)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240606212728_u8ve GAAGGCTACATGGAGCCCTCACAATAAAAATG(+ATATAT)GTACCTTTTTTTTTTGAGACAGGCTCTTACT NGG 10 8 17 8 24 0 100 5 10
    240606212726_8otk GGGCTAGTGTACAAGCACAGCCAGAGTCGTCG(+ATATAT)GTACCAGCACTTCTCTATTTACTCTATTGGA NGG 10 8 17 8 24 0 100 5 10
    240606212724_zq4e TCCGCTCCAATAGAGTAAATAGAGAAGTGCTG(+ATATAT)GTACCGACGACTCTGGCTGTGCTTGTACACT NGG 10 8 17 8 24 0 100 5 10
    240606212722_347c TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+ATATAT)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240606212719_poci GAAGGCTACATGGAGCCCTCACAATAAAAATG(+ATATAT)GTACCTTTTTTTTTTGAGACAGGCTCTTACT NGG 10 8 17 8 24 0 100 5 10
    240606212718_jk4t GGGCTAGTGTACAAGCACAGCCAGAGTCGTCG(+ATATAT)GTACCAGCACTTCTCTATTTACTCTATTGGA NGG 10 8 17 8 24 0 100 5 10
    240606212717_60lo TCCGCTCCAATAGAGTAAATAGAGAAGTGCTG(+ATATAT)GTACCGACGACTCTGGCTGTGCTTGTACACT NGG 10 8 17 8 24 0 100 5 10