Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212715_wa2g TTCAGGATAGTATGGGAACAAAGAAAGAGAAG(+ATATAT)GTACCTCTATCCCTGTATCTCCTTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606212714_ci6u GGTGTTCCTTTGAAGGAGATACAGGGATAGAG(+ATATAT)GTACCTTCTCTTTCTTTGTTCCCATACTATC NGG 10 8 17 8 24 0 100 5 10
    240606212712_vxon GCAGGCACAAAACCTTTACTCCAATTCCTTGG(+ATATAT)GATCCTTCATTCTATCCCTCTCCAGAACTCT NGG 10 8 17 8 24 0 100 5 10
    240606212711_sr2n TCTCTAGAGTTCTGGAGAGGGATAGAATGAAG(+ATATAT)GATCCCAAGGAATTGGAGTAAAGGTTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606212709_1yye GCAGCAGGCAGGTGGAAGGTAACCACTGTCAG(+ATATAT)GATCCCCTGAACTGCACGTGTCCTTCCCTAC NGG 10 8 17 8 24 0 100 5 10
    240606212708_4bo8 CCAAAGTAGGGAAGGACACGTGCAGTTCAGGG(+ATATAT)GATCCTGACAGTGGTTACCTTCCACCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606212706_q4c9 AGATATGCTTTAAAGAGAGGCCTTAATTTAAG(+ATATAT)GTACCGCCATCTTGGCTCACAAGAGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240606212704_maki TCTGAACTGGGCTCTTGTGAGCCAAGATGGCG(+ATATAT)GTACCTTAAATTAAGGCCTCTCTTTAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606212702_wzk9 GTGACACATTGAAGAAGATGAGTGAGATCCAG(+ATATAT)GATCCCCACTCTATCCACACATCCAAGATCA NGG 10 8 17 8 24 0 100 5 10
    240606212700_ju6h CCAGTTGATCTTGGATGTGTGGATAGAGTGGG(+ATATAT)GATCCTGGATCTCACTCATCTTCTTCAATGT NGG 10 8 17 8 24 0 100 5 10