Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212603_h9ow ACAAGTACAGTTTCCAGTGCCTCCCTGCTGTG(+ATATAT)GTACCTGAAAAGTCTGAATGCAAGTAGTATG NGG 10 8 17 8 24 0 100 5 10
    240606212601_998y ATAGTCATACTACTTGCATTCAGACTTTTCAG(+ATATAT)GTACCACAGCAGGGAGGCACTGGAAACTGTA NGG 10 8 17 8 24 0 100 5 10
    240606212600_9gq7 AAAAATGTTCCTGCAAAGGACATCCACTCCTG(+ATATAT)GATCCTGATGCCATTCACACAATGAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240606212558_v8pa GCAGCACTATCTCATTGTGTGAATGGCATCAG(+ATATAT)GATCCAGGAGTGGATGTCCTTTGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606212556_ebj3 TGGGGTGTTTCCTTCCGGCAGAAGCCACCAGG(+ATATAT)GATCCAAGCTTCATGTGGTTGGCTCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606212555_gsmp TTCAGTCTGGGGAGCCAACCACATGAAGCTTG(+ATATAT)GATCCCTGGTGGCTTCTGCCGGAAGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606212554_ohav TTCAAGTCACAAAGTTACTGTCCCACGGAAAG(+ATATAT)GATCCCAATCTCCTGCAGGGACATTATGAGT NGG 10 8 17 8 24 0 100 5 10
    240606212552_h1vy ACCTTACTCATAATGTCCCTGCAGGAGATTGG(+ATATAT)GATCCTTTCCGTGGGACAGTAACTTTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606212550_l2rf CCTTGACCATTATAATTTAAATGTGTTTGGAG(+ATATAT)GTACCAGGCTCATGGGCATAATTTATAATAT NGG 10 8 17 8 24 0 100 5 10
    240606212548_k3nz AGTTGATATTATAAATTATGCCCATGAGCCTG(+ATATAT)GTACCTCCAAACACATTTAAATTATAATGGT NGG 10 8 17 8 24 0 100 5 10