Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212505_0b7l CATGCCATTTTAGGGCCATTGTGATTCTCAGG(+ATATAT)GATCCTAGGTAAAAATCCTGAATCTTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606212503_dyd4 TCTTACCCAAAAGATTCAGGATTTTTACCTAG(+ATATAT)GATCCCTGAGAATCACAATGGCCCTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606212502_nhva TCTTGCTTGTCAACTTCAGAGATAGCTGGAAG(+ATATAT)GATCCTGAGACTGTATGGCAGGAGAGTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606212500_ktig AACCCCTCAACTCTCCTGCCATACAGTCTCAG(+ATATAT)GATCCTTCCAGCTATCTCTGAAGTTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606212459_z6uy AAAAGCATGAGATTTGCATCCCAGCTGTCCTG(+ATATAT)GATCCAGGCTGTGAGGTTTTGAGGATTCTTA NGG 10 8 17 8 24 0 100 5 10
    240606212456_c7ya GTGAGTAAGAATCCTCAAAACCTCACAGCCTG(+ATATAT)GATCCAGGACAGCTGGGATGCAAATCTCATG NGG 10 8 17 8 24 0 100 5 10
    240606212454_lpg2 TGGTTCATCTGTTTGAGAGCCTTTGGGAAGGG(+ATATAT)GATCCTGTTTGCTGGAAAATGTTGGGAGATC NGG 10 8 17 8 24 0 100 5 10
    240606212453_3jqv TCTGGGATCTCCCAACATTTTCCAGCAAACAG(+ATATAT)GATCCCCTTCCCAAAGGCTCTCAAACAGATG NGG 10 8 17 8 24 0 100 5 10
    240606212451_sqmh TAATTCTGGAACCCCAGGGAAGCCAAGATCAG(+ATATAT)GATCCTGTTGATCTCTTGTTTTCTAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606212450_5vgu GAAGAAGGCCTTAGAAAACAAGAGATCAACAG(+ATATAT)GATCCTGATCTTGGCTTCCCTGGGGTTCCAG NGG 10 8 17 8 24 0 100 5 10