Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213553_44w6 CTGGGACCTCATCCCAGCAGTGTGGGCTTATG(+ATATAT)GTACCCGCTGGGCATGCTGGGGTTATCCAGT NGG 10 8 17 8 24 0 100 5 10
    240606213552_2ikr GATGGATAGGATGGATGCACCAACAGCTGTTG(+ATATAT)GTACCTTGTTTTTCCTTCAACTGTTGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606213550_luaf AGTCAATACCCAACAGTTGAAGGAAAAACAAG(+ATATAT)GTACCAACAGCTGTTGGTGCATCCATCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606213548_fnjv CTCGCATACTTTGACTTCATTTCTATTCCTGG(+ATATAT)GATCCACCACCCTCTTCTCTCAGTTGACTGC NGG 10 8 17 8 24 0 100 5 10
    240606213547_g7w8 ATGCAGCAGTCAACTGAGAGAAGAGGGTGGTG(+ATATAT)GATCCCAGGAATAGAAATGAAGTCAAAGTAT NGG 10 8 17 8 24 0 100 5 10
    240606213545_pd4l TGCCTCCAGCAGGGGATGCTGGGGGTGGAGAG(+ATATAT)GTACCTCTGGCCCTAAGTCACACTGGGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606213543_7s6g GCTCCTGCACCCAGTGTGACTTAGGGCCAGAG(+ATATAT)GTACCTCTCCACCCCCAGCATCCCCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606213542_nsuh CTGGGACAAGAGTCTGGGCAGTCATGGCATTG(+ATATAT)GTACCAATGCCCGGCTGCCTGTCCTATTGAA NGG 10 8 17 8 24 0 100 5 10
    240606213541_hz45 GCTACTTCAATAGGACAGGCAGCCGGGCATTG(+ATATAT)GTACCAATGCCATGACTGCCCAGACTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606213539_wrkb AGGACCCCCTCTTCCCCCAGAGGGGTCTCCTG(+ATATAT)GATCCCCTTTCCTGTGAATGGAGGCTCCAAT NGG 10 8 17 8 24 0 100 5 10