Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212417_rtlf GTTGGGCAACACTTAGATGGAAAGTTTGTGGG(+ATATAT)GTACCTGTCCAATCATTTTCCTTCCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606212416_rghn CCCTAGCCTAGGGCTTCGACATTGCATGCTGG(+ATATAT)GATCCAGCTCCTACCCTTCCTGCACTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606212414_f7mt TGGGGAAGGGAGTGCAGGAAGGGTAGGAGCTG(+ATATAT)GATCCCAGCATGCAATGTCGAAGCCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606212413_rqn4 CTAACCACTGCCCTCCTGGAACTGCTGTGCTG(+ATATAT)GATCCAAAGGCCTCCTCACCAGGAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606212411_py5q GGGCCAGAGCCTTCCTGGTGAGGAGGCCTTTG(+ATATAT)GATCCAGCACAGCAGTTCCAGGAGGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606212409_omxt ATAGGTGCTGGAGAAGCGGGGACCGCAGTGGG(+ATATAT)GTACCATCAGGCTCGGGGTAAGGTAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606212408_wvb8 TTACCCTTTTCTACCTTACCCCGAGCCTGATG(+ATATAT)GTACCCCACTGCGGTCCCCGCTTCTCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606212406_vk66 CTCCTCTACACAGCAGCAACTAAAATGATCAG(+ATATAT)GATCCTTCACTGGAGCCCTAGAGACTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606212404_3l0m ATCTTCTGGGAGTCTCTAGGGCTCCAGTGAAG(+ATATAT)GATCCTGATCATTTTAGTTGCTGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606212403_j7wv CATACCTAGGAGCAAGCACTGATCGCTGAGCG(+ATATAT)GTACCAAGAGGAGCCTCTCCTTTTACTGGAT NGG 10 8 17 8 24 0 100 5 10