Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212345_sdz8 CCAGGTGTCTTCTCAAACGGAGTAAGCGCCTG(+ATATAT)GATCCAAATGGTAGACTACTTGACCCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240606212343_6gv4 GTCAGGAGCCCAATTCCTGGGAGCAGTAGGGG(+ATATAT)GATCCAGGACCTCCAGTCAGCCCACACACCT NGG 10 8 17 8 24 0 100 5 10
    240606212342_7u9i CGCTGAGGTGTGTGGGCTGACTGGAGGTCCTG(+ATATAT)GATCCCCCTACTGCTCCCAGGAATTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606212340_3082 TGCCCAGGGGCGCGCGCTCTTAATTGACTCGG(+ATATAT)GATCCTTGGAACGATCCCCTTCTTGCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606212338_7w9i TGTCATAAAGGCAAGAAGGGGATCGTTCCAAG(+ATATAT)GATCCCGAGTCAATTAAGAGCGCGCGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606212337_p7t6 CTTTGCCAGCCCCCTACCCCTTTATTATCTCG(+ATATAT)GATCCCATCTTCTCCAGCAGCAGGCACCAAG NGG 10 8 17 8 24 0 100 5 10
    240606212335_b70t CTCGCCTTGGTGCCTGCTGCTGGAGAAGATGG(+ATATAT)GATCCGAGATAATAAAGGGGTAGGGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606212334_nqcd ACTGTTGTTGTCTTCAGACACACCAGAAGAGG(+ATATAT)GATCCCATTATGGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606212332_417d ACCACATGGTGGCTCACAACCATCCATAATGG(+ATATAT)GATCCCTCTTCTGGTGTGTCTGAAGACAACA NGG 10 8 17 8 24 0 100 5 10
    240606212330_v5xf TTTCTATGTAATCCAAGTGCCTCCTCCGGCTG(+ATATAT)GTACCAGGATGGGAACAGAAGCCGGTTTATC NGG 10 8 17 8 24 0 100 5 10