Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212310_jbnq TTCCTGGCTCAGTCTTTTCTTGGGGGGCAGTG(+ATATAT)GTACCCCAGTATCCACTTGGCAAATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606212309_wx6q CACAGGGCCCAGATGGTGGGTAACTGAGGCAG(+ATATAT)GATCCAGACTTGAGACACACGGTTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606212307_lvhs AGCCTTGTTTCCAACCGTGTGTCTCAAGTCTG(+ATATAT)GATCCTGCCTCAGTTACCCACCATCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606212305_dif4 GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+ATATAT)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606212304_y6n8 GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+ATATAT)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606212302_276o TGTAAAATTCCAGTGACTCAAATGGCTTTAGG(+ATATAT)GATCCCCTGGAGACGGTTCAGGAAGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606212259_vr94 CCAACAACTCCCTTCCTGAACCGTCTCCAGGG(+ATATAT)GATCCCTAAAGCCATTTGAGTCACTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240606212258_otme GCAAAACTAACCTAAGGCTAAGTGACCACAGG(+ATATAT)GATCCTCTGGGTGAACAGTGAGGACTATAAC NGG 10 8 17 8 24 0 100 5 10
    240606212255_cfqp CTGTGGTTATAGTCCTCACTGTTCACCCAGAG(+ATATAT)GATCCCTGTGGTCACTTAGCCTTAGGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606212253_ne6b CTCGGTGCATAACCTTGATCGTGGAGCCTCAG(+ATATAT)GATCCCAAAACAGGATGTTTTGGGTGTCAAT NGG 10 8 17 8 24 0 100 5 10