Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606213640_f188 ATCATTGGACATTCTTGAGCACTGCAGGTAGG(+ATATAT)GTACCCTTCATTCACCACCCCCACACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606213638_6xly TTAATCCCAGCACTAAGGAGGCAGAGCAGGCG(+ATATAT)GATCCCAGTGAGTTTGAAGCCTGAGCTATAG NGG 10 8 17 8 24 0 100 5 10
    240606213637_x6j3 TCATCCTATAGCTCAGGCTTCAAACTCACTGG(+ATATAT)GATCCGCCTGCTCTGCCTCCTTAGTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606213635_gty4 GGACAGAAGCAGTTTGAGCAGGTGTCTATATG(+ATATAT)GTACCCTGCATCCGGGGCCCTTTGGGAACTG NGG 10 8 17 8 24 0 100 5 10
    240606213633_pi6e CCGGCCAGTTCCCAAAGGGCCCCGGATGCAGG(+ATATAT)GTACCATATAGACACCTGCTCAAACTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606213631_3yni CACATTTCTAAAAATCTTTCAATGACAAGTTG(+ATATAT)GTACCCTTTAGCTAACTAAAGCTAAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606213630_jau6 CCTTCCACCTTTTAGCTTTAGTTAGCTAAAGG(+ATATAT)GTACCAACTTGTCATTGAAAGATTTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606213628_tsru TCCTCCTATTCCTCCTGTGAGTTAGTGGCAGG(+ATATAT)GTACCAACCCACTGGAGGGCAACAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606213627_bp2l CCACACCCTCCCTGTTGCCCTCCAGTGGGTTG(+ATATAT)GTACCCTGCCACTAACTCACAGGAGGAATAG NGG 10 8 17 8 24 0 100 5 10
    240606213626_x1az GAAGGTCAGACAACAGCTTCCCTACTTGCCAG(+ATATAT)GTACCTGCACTGGGGGGCCCCGGGCCACAGG NGG 10 8 17 8 24 0 100 5 10