Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606212449_z3q3 TACTTTGTCAGAGTTCTGACTGTCAGTCACAG(+ATATAT)GATCCTATTAGTGTTGCAAGTCTAGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606212448_65ks CACTCTACTTGCTAGACTTGCAACACTAATAG(+ATATAT)GATCCTGTGACTGACAGTCAGAACTCTGACA NGG 10 8 17 8 24 0 100 5 10
    240606212447_lccp GTAATCCCCTCGTTGGGAGGTGGAGACAAGAG(+ATATAT)GATCCCTGAGGCTTGCTGGGCTGCTAATATA NGG 10 8 17 8 24 0 100 5 10
    240606212444_9e9k TGAACTATATTAGCAGCCCAGCAAGCCTCAGG(+ATATAT)GATCCTCTTGTCTCCACCTCCCAACGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606212443_f5xc TCTACACCGAGTTACTGATAAACCTCATCAAG(+ATATAT)GTACCTGTTCTGTCAGGTGGTGTCCTAATCT NGG 10 8 17 8 24 0 100 5 10
    240606212442_7367 AGAGCAGATTAGGACACCACCTGACAGAACAG(+ATATAT)GTACCTTGATGAGGTTTATCAGTAACTCGGT NGG 10 8 17 8 24 0 100 5 10
    240606212441_0lk4 TGTCCACACATCCCTCTCTAAGCCCAGGATAG(+ATATAT)GTACCAATATAGGTAACTCTGCATTTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606212439_58xk CTTATGGGAAAAATGCAGAGTTACCTATATTG(+ATATAT)GTACCTATCCTGGGCTTAGAGAGGGATGTGT NGG 10 8 17 8 24 0 100 5 10
    240606212438_gbq9 TACACCTCTAGGAACTGAGGCAGCTTCAGAAG(+ATATAT)GTACCTGCCAGATGTTGCTGTGGGAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606212437_x7az AAGGCCATGCTTCCCACAGCAACATCTGGCAG(+ATATAT)GTACCTTCTGAAGCTGCCTCAGTTCCTAGAG NGG 10 8 17 8 24 0 100 5 10