Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180600_mdjr TGTTTCAAGAGATGGTTGCCAGCTGACCGCAG(+AAAAAA)GTACCTTAATAAAGACCATTGCATCTTGATG NGG 10 8 17 8 24 0 100 5 10
    240606180559_q6v3 GTAGCGTCTCTGGAGGCCAGAAGATGGCATTG(+AAAAAA)GATCCCCGGAACTGGAGTCACAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606180556_kdu3 TGGAGCTCACAACTTGTGACTCCAGTTCCGGG(+AAAAAA)GATCCAATGCCATCTTCTGGCCTCCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606180555_ulnx CAAACTCACAATCCTCCTGCCTCAGGCACTTG(+AAAAAA)GATCCTGGAATTGCAGTCAAAGAGCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240606180553_hre4 GGCTGGAAGTAGCTCTTTGACTGCAATTCCAG(+AAAAAA)GATCCAAGTGCCTGAGGCAGGAGGATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606180551_248d CTGCAGCCCTCGGGCGCCTGGCTCTGACAGGG(+AAAAAA)GATCCCTGGAGGAGGAAGAGGACGCCGCTAC NGG 10 8 17 8 24 0 100 5 10
    240606180549_6d70 TCAGCGTAGCGGCGTCCTCTTCCTCCTCCAGG(+AAAAAA)GATCCCCTGTCAGAGCCAGGCGCCCGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606180548_qhzb TTACTCTTACTCCAAGCTTCCAGGTCACTGTG(+AAAAAA)GTACCCTGGCAAACAGCTGAACCAGGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606180546_5463 CCCTCTGCAGCCTGGTTCAGCTGTTTGCCAGG(+AAAAAA)GTACCACAGTGACCTGGAAGCTTGGAGTAAG NGG 10 8 17 8 24 0 100 5 10
    240606180544_rokd GGCTGGGGCGGGCATCACCAGGATAGCGCCTG(+AAAAAA)GATCCTGGTGGGCACGGGGTCACTGAGCTGC NGG 10 8 17 8 24 0 100 5 10