Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180525_7nhy TATGTAGCAGATGTACAGGCTGGTCTTCATCG(+AAAAAA)GATCCCCCAACAACCGGAGCTGGTCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240606180524_l5n7 GAGCTTGCGCTTGCTTGCTAAACCTCTAACAG(+AAAAAA)GTACCTGGGGATGATGGTGCACACCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240606180521_hp3l TTAGGGTTATAGGTGTGCACCATCATCCCCAG(+AAAAAA)GTACCTGTTAGAGGTTTAGCAAGCAAGCGCA NGG 10 8 17 8 24 0 100 5 10
    240606180520_3zzn GGACAAAAAAAAAAGAAAAGAAAAGAAAAAAG(+AAAAAA)GATCCCCCCCTTCTGTTTTTGTTCCTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606180517_2u9g AGAAATCACGAGGAACAAAAACAGAAGGGGGG(+AAAAAA)GATCCTTTTTTCTTTTCTTTTCTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606180515_67wv AGTTGATAAGTTTTCTGATATTCTGATATTAG(+AAAAAA)GTACCACGGATGCAGGAATATTACTTGATAT NGG 10 8 17 8 24 0 100 5 10
    240606180513_s6fo AGCCAATATCAAGTAATATTCCTGCATCCGTG(+AAAAAA)GTACCTAATATCAGAATATCAGAAAACTTAT NGG 10 8 17 8 24 0 100 5 10
    240606180511_po7c GCACCTGCTTGGCTGTGACTCAGGTCCCAGGG(+AAAAAA)GATCCAGATCCCTTCTCTGACCTTTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606180509_q73r CTGGTGCCAGCAAAGGTCAGAGAAGGGATCTG(+AAAAAA)GATCCCCTGGGACCTGAGTCACAGCCAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606180508_kwzh GGTCTTTTGAGAGATTTATTGAATAAGAACAG(+AAAAAA)GTACCAGCGGGACACTGAAACAGTTATGCTG NGG 10 8 17 8 24 0 100 5 10