Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180542_csju GGAAGGCAGCTCAGTGACCCCGTGCCCACCAG(+AAAAAA)GATCCAGGCGCTATCCTGGTGATGCCCGCCC NGG 10 8 17 8 24 0 100 5 10
    240606180540_taon AGTGGAGACTCAGCTCCCTGAATCTGGAGCCG(+AAAAAA)GATCCGTAGCCCCTGCCCTACACTCATTCTG NGG 10 8 17 8 24 0 100 5 10
    240606180539_hmdl CCCTTCAGAATGAGTGTAGGGCAGGGGCTACG(+AAAAAA)GATCCGGCTCCAGATTCAGGGAGCTGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606180537_f1hx CCATGGCCCCTTGGTATCTGTGGGATGCTGGG(+AAAAAA)GATCCGAATCCTGGCCCTCATGCTTGGACAG NGG 10 8 17 8 24 0 100 5 10
    240606180536_vjan GCTTGCTGTCCAAGCATGAGGGCCAGGATTCG(+AAAAAA)GATCCCCAGCATCCCACAGATACCAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606180534_5dfk AAGATCAGCACTCCTGCTTGCCTGACTTGCTG(+AAAAAA)GATCCCAAGGGCACTCACTGAACCACCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606180531_dygl TTATGGGAGGGTGGTTCAGTGAGTGCCCTTGG(+AAAAAA)GATCCAGCAAGTCAGGCAAGCAGGAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606180530_pn48 TCTGCAGTCTGTCTCCGGCCTGGGGACACATG(+AAAAAA)GTACCTTCAAAGTAGTTGGAATCTGAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606180529_svq5 AGCGTGATGCTCAGATTCCAACTACTTTGAAG(+AAAAAA)GTACCATGTGTCCCCAGGCCGGAGACAGACT NGG 10 8 17 8 24 0 100 5 10
    240606180526_3f1y AGTCAGGGTAGGACCAGCTCCGGTTGTTGGGG(+AAAAAA)GATCCGATGAAGACCAGCCTGTACATCTGCT NGG 10 8 17 8 24 0 100 5 10