Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181852_oueh GTTTGTTTGTTTGAATAAACTAAAAATGCCTG(+AGGACA)GTACCTAGGACGTAATCTAGAACTGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240613181850_p8ec CCCCAAAATGGCAGTTCTAGATTACGTCCTAG(+AGGACA)GTACCAGGCATTTTTAGTTTATTCAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240613181848_q0xl ATCTCAACAATGTGTGGGTCAAGCAGTAGCTG(+AGGACA)GTACCTTACTCCTTCTCTTGTGCCAACCCCC NGG 10 8 17 8 24 0 100 5 10
    240613181847_r562 TATTTGGGGGTTGGCACAAGAGAAGGAGTAAG(+AGGACA)GTACCAGCTACTGCTTGACCCACACATTGTT NGG 10 8 17 8 24 0 100 5 10
    240613181846_e39q GCATTCAGAGCTAACTTCTGAAAACAGGAATG(+AGGACA)GATCCCACAAGACTTTGCTTTGGGTCATAAG NGG 10 8 17 8 24 0 100 5 10
    240613181844_0phs GGTAGCTTATGACCCAAAGCAAAGTCTTGTGG(+AGGACA)GATCCATTCCTGTTTTCAGAAGTTAGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613181843_ai2n GAGTCTTTGAGCTTGGCTTTTAACTCTTTAAG(+AGGACA)GTACCATTGGGAACATGATTTTTTATTAATC NGG 10 8 17 8 24 0 100 5 10
    240613181841_05wj CCTCTGATTAATAAAAAATCATGTTCCCAATG(+AGGACA)GTACCTTAAAGAGTTAAAAGCCAAGCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613181839_1h9n TTCAGAAGTCACTCACTCATGTGCTTAGTTAG(+AGGACA)GATCCAAGAACCAGCAAAGACCCTCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240613181838_rmo7 TTTACTGTCCTGAGGGTCTTTGCTGGTTCTTG(+AGGACA)GATCCTAACTAAGCACATGAGTGAGTGACTT NGG 10 8 17 8 24 0 100 5 10