Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181821_rs23 ACAAATGAGTCCAGGAAGATGGAGCCCTTTAG(+AGGACA)GATCCATCAAGCCCCAAATGAAAGCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613181820_s3qk AATAAAAGCAAGCTTTCATTTGGGGCTTGATG(+AGGACA)GATCCTAAAGGGCTCCATCTTCCTGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240613181818_vy8b TTCCATTGGGGTGGCCCTACTCCGGCCCTAAG(+AGGACA)GATCCCCAAGGCACAGCTAATGGTTCCTTAC NGG 10 8 17 8 24 0 100 5 10
    240613181816_4l1n CAAGGGTAAGGAACCATTAGCTGTGCCTTGGG(+AGGACA)GATCCTTAGGGCCGGAGTAGGGCCACCCCAA NGG 10 8 17 8 24 0 100 5 10
    240613181814_4klh ATAAGAGCATCCCTGCCCTGCCTGAGTCAGGG(+AGGACA)GATCCACAGCCCCATTGGGACAAGAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240613181813_zl2r GGTCCCTGCATTCTTGTCCCAATGGGGCTGTG(+AGGACA)GATCCCCTGACTCAGGCAGGGCAGGGATGCT NGG 10 8 17 8 24 0 100 5 10
    240613181811_hdvu GACCCCCAGCACCTTGGCCCACCAGAGGCTGG(+AGGACA)GTACCCAGGCAAGGGATGTTTCAAGGCACAT NGG 10 8 17 8 24 0 100 5 10
    240613181809_3e43 ATTGTATGTGCCTTGAAACATCCCTTGCCTGG(+AGGACA)GTACCCAGCCTCTGGTGGGCCAAGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613181807_xti1 ACTGTGGGTGCTACACACAAACACTTGGGCAG(+AGGACA)GTACCCTACTGTCCTATAACTTCCCTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240613181805_85u8 GTCTAAAGGCAGGGAAGTTATAGGACAGTAGG(+AGGACA)GTACCTGCCCAAGTGTTTGTGTGTAGCACCC NGG 10 8 17 8 24 0 100 5 10