Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181714_yilu CTGGTGGCTGGTCTGTCATTCTGTTTACCCTG(+AGGACA)GATCCTCTTTATGTCATGGCTGACAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240613181712_csk7 TAGCTACTAATTGTCAGCCATGACATAAAGAG(+AGGACA)GATCCAGGGTAAACAGAATGACAGACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613181711_a0gv TGAGCTGAGTGAGGGAGCGTCCAGCTTGGTTG(+AGGACA)GATCCTGATTTAGGAAGTGTGGCTTTCCGAC NGG 10 8 17 8 24 0 100 5 10
    240613181710_fvbp CTCAAGTCGGAAAGCCACACTTCCTAAATCAG(+AGGACA)GATCCAACCAAGCTGGACGCTCCCTCACTCA NGG 10 8 17 8 24 0 100 5 10
    240613181708_m7hq GTGGCAATAGTAGCCACCTGTCCTCCCAGGAG(+AGGACA)GATCCCAAGCCCTCCATGGAGTGGCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613181706_7nv3 CCTCATTCTGAGCCACTCCATGGAGGGCTTGG(+AGGACA)GATCCTCCTGGGAGGACAGGTGGCTACTATT NGG 10 8 17 8 24 0 100 5 10
    240613181704_djcw GTCGATCTAGCCATTCAATGCTGTCTTTCCTG(+AGGACA)GATCCATCTTAAAAGCCGCAGCTCACTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613181703_y5nb AATGCAGAAAGTGAGCTGCGGCTTTTAAGATG(+AGGACA)GATCCAGGAAAGACAGCATTGAATGGCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613181701_av7c TTCCTGCCTAAACGCAAACAGACCAAAGCCCG(+AGGACA)GATCCTCCTGCTGGGCCCATTCCCACACAGC NGG 10 8 17 8 24 0 100 5 10
    240613181659_yc06 TCCCTGCTGTGTGGGAATGGGCCCAGCAGGAG(+AGGACA)GATCCGGGCTTTGGTCTGTTTGCGTTTAGGC NGG 10 8 17 8 24 0 100 5 10