Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181641_avzf TACTGAATTTTTGTCTCATTCACATATTCCAG(+AGGACA)GTACCAACCACTGTGTGTGACCTACTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613181640_qi40 TAGTTTCTTTAGTAGGTCACACACAGTGGTTG(+AGGACA)GTACCTGGAATATGTGAATGAGACAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240613181638_3rb5 GCTGGGAGGGCCGTGTACAGCCCTTGGTCTGG(+AGGACA)GATCCATGTGTTGGTTGAGAAATCATTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613181637_n137 ACACTCTGGAATGATTTCTCAACCAACACATG(+AGGACA)GATCCCAGACCAAGGGCTGTACACGGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613181635_r8il TGTCCCCTCCCTGGTCTTATGCCACTTGCCAG(+AGGACA)GTACCCTCAGAGCTGTGGGGTGCCTTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613181633_88hk ACGCAGAACTAAGGCACCCCACAGCTCTGAGG(+AGGACA)GTACCTGGCAAGTGGCATAAGACCAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613181631_3mn8 AGGCTTAGAACTTGAACTCGCCCTGACCTCTG(+AGGACA)GATCCTGTTCTTCAAGGAAGCAAAACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613181630_6apn TAAGGATTTGGTTTTGCTTCCTTGAAGAACAG(+AGGACA)GATCCAGAGGTCAGGGCGAGTTCAAGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240613181629_wmcx GCCAGCCCAGAGCCGGGCCACTCCCCTTTCTG(+AGGACA)GTACCAAGAAAAGCTTTCCTTGATGACCAGT NGG 10 8 17 8 24 0 100 5 10
    240613181628_bn55 AGGGCACTGGTCATCAAGGAAAGCTTTTCTTG(+AGGACA)GTACCAGAAAGGGGAGTGGCCCGGCTCTGGG NGG 10 8 17 8 24 0 100 5 10