Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181626_7d57 TCAGTGCCGCTCAGCCTTGGCCCTGTGTGGCG(+AGGACA)GATCCTGACCTAGAGAACAGGTGTGGGGACA NGG 10 8 17 8 24 0 100 5 10
    240613181625_shqg TGCAGTGTCCCCACACCTGTTCTCTAGGTCAG(+AGGACA)GATCCGCCACACAGGGCCAAGGCTGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240613181624_d5vs GCCTTTGCCTACAGAGACATGAGGCTGAGCTG(+AGGACA)GATCCTGACAATACTGTCCATTTGCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240613181623_t9o7 AGGGCACTCAGGCAAATGGACAGTATTGTCAG(+AGGACA)GATCCAGCTCAGCCTCATGTCTCTGTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613181621_5a28 AACCCCCAAGAGTGAGTCTGTATTTCTTTGTG(+AGGACA)GATCCTAAGGAACTTAGATTAGTACCTTAGC NGG 10 8 17 8 24 0 100 5 10
    240613181619_rden CTGATGCTAAGGTACTAATCTAAGTTCCTTAG(+AGGACA)GATCCACAAAGAAATACAGACTCACTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613181617_7ply GTGCCGGTGTAGGGGCAGGATCTCTGGACCCG(+AGGACA)GATCCCACGCCAGGGCTTTCGCTCTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613181615_21ub GAGCATCAGCAAGAGCGAAAGCCCTGGCGTGG(+AGGACA)GATCCGGGTCCAGAGATCCTGCCCCTACACC NGG 10 8 17 8 24 0 100 5 10
    240613181614_38sy CTCTCCCACCCGGCTCTAGGCCAAAAGGCTCG(+AGGACA)GTACCTCCTTACTGGGAGCCACGTAAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613181612_5dyv AAGGTTCCATTTACGTGGCTCCCAGTAAGGAG(+AGGACA)GTACCGAGCCTTTTGGCCTAGAGCCGGGTGG NGG 10 8 17 8 24 0 100 5 10