Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181611_g8e1 TGCAGCTAGCTGGTGCCACAGACGCAGCTCTG(+AGGACA)GTACCTCGATTATTTGTTTTTAATATATATC NGG 10 8 17 8 24 0 100 5 10
    240613181610_ugjp ATGCAGATATATATTAAAAACAAATAATCGAG(+AGGACA)GTACCAGAGCTGCGTCTGTGGCACCAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240613181608_zyus GGCAAGTATGCAACTGGGAAGCTGCCATCAGG(+AGGACA)GATCCTCCCCTGCCCTACACAGCCAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613181606_2fom CGCCTGGAGCCTGGCTGTGTAGGGCAGGGGAG(+AGGACA)GATCCCTGATGGCAGCTTCCCAGTTGCATAC NGG 10 8 17 8 24 0 100 5 10
    240613181605_jbsc CCTAGAGGCCCTTCCCCGCTGGCGGGTTCATG(+AGGACA)GATCCTTCTAATAAACTTATGTGTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613181604_1b6c GGAAGGAGGAGGACACATAAGTTTATTAGAAG(+AGGACA)GATCCATGAACCCGCCAGCGGGGAAGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613181602_8kav GCCCAGAGCTTGTTCTAAACTGCGAAGTCCAG(+AGGACA)GTACCTAGCCCCACACCTGCTCCTCCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613181600_m2e9 ACCCCAGGAAGGAGGAGCAGGTGTGGGGCTAG(+AGGACA)GTACCTGGACTTCGCAGTTTAGAACAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613181559_jdfv TCTGGGAAGACTGGGCTAGAGGAAAGAGACTG(+AGGACA)GTACCAGTCTTGGCAGGAGGGTGGGGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240613181558_00kp CAGGACCTTGCCCCACCCTCCTGCCAAGACTG(+AGGACA)GTACCAGTCTCTTTCCTCTAGCCCAGTCTTC NGG 10 8 17 8 24 0 100 5 10